IFN-, but not IP-10, MCP-2 or IL-2 response to RD1 selected peptides associates to active tuberculosis.

Goletti, D and Raja, Alamelu and Kabeer, Basirudeen S A and Rodrigues, C and Sodha, A and Butera, O and Carrara, S and Vernet, G and Loguet, C and Ippolito, G and Thangaraj, S and Leportier, M and Girardi, E and Lagrange, P H (2010) IFN-, but not IP-10, MCP-2 or IL-2 response to RD1 selected peptides associates to active tuberculosis. Journal of Infection, 61 (2). pp. 133-143. ISSN 0163-4453

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Abstract

Summary Objectives: To evaluate whether in vitro response to Mycobacterium tuberculosis RD1 peptides selected by computational analysis, measured by IFN-g, IP-10, MCP-2 or IL-2 production, is associated with active tuberculosis (TB) in a country with a high incidence of TB. Methods: 129 individuals were prospectively enrolled, 41 with active-pulmonary TB and 88 without (household contacts and community controls). A whole blood assay based on RD1 selected peptides was performed. Soluble factors were evaluated by ELISA in plasma harvested at day1-post-culture. Enrolled individuals were also tested by QuantiFERON TB-Gold In tube (QFT-IT) and tuberculin skin tests (TST). Results: IFN-g response to RD1 selected peptides was significantly higher in active TB patients than in household contacts and community controls. IP-10 and MCP-2 response did not differ between active TB patients and household contacts, although it was higher in these groups compared to community controls; conversely IL-2 response did not differ among the three groups. When IFN-g response to RD1 selected peptides was scored based on receiver-operator- characteristic analysis, active TB was predicted with 68% sensitivity and 86% specificity.

Affiliation:	ICMR-National Institute for Research in Tuberculosis
Item Type:	Article
Uncontrolled Keywords:	Tuberculosis; LTBI; IGRA; RD1 peptides; IP-10; MCP-2; IL-2
Subjects:	Tuberculosis > Laboratory Research > Immunological
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	26 May 2022 10:23
Last Modified:	26 May 2022 10:23
URI:	http://eprints.nirt.res.in/id/eprint/1055

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