Fast track method for the identification of multi-drug resistant tuberculosis on direct clinical specimen using combined drug media

Ramachandran, Ranjani and Lakshmi, R and Ravikumar, D and Devika, K and Rahman, Fathima and Wares, D F (2011) Fast track method for the identification of multi-drug resistant tuberculosis on direct clinical specimen using combined drug media. Asian Pacific Journal of Tropical Disease, 1 (1). pp. 47-49. ISSN 2222-1808

Archive 201110.pdf - Published Version Restricted to Registered users only Download (643kB) | Request a copy

Abstract

Objective To combine isoniazid (INH) and rifampicin (RIF) in a single media to detect and evaluate multi-drug resistant Mycobacterium tuberculosis (MDR-TB) strains using clinical specimens by direct and indirect drug susceptibility testing (DST). Methods Drug susceptibility testing for INH and RIF using individual and combined drug media was performed by minimum inhibitory concentration (MIC) method on direct clinical specimens. Results The combined drug media showed complete concordance with individual drug media in the detection of MDR-TB by direct DST method and 89% efficiency with indirect DST method. Susceptibility results were available by 3 weeks after the receipt of clinical specimen using direct DST on combined drug media. Conclusions Combined drug media can be used as a fast track method in large scale studies warranting detection of MDR-TB.

Affiliation:	ICMR-National Institute for Research in Tuberculosis
Item Type:	Article
Uncontrolled Keywords:	Drug susceptibility, testingMycobacterium tuberculosis, Isoniazid, Rifampicin, Multi–drug resistance
Subjects:	Tuberculosis > Clinical Research
Divisions:	Clinical Research
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	16 Jun 2022 07:46
Last Modified:	16 Jun 2022 07:46
URI:	http://eprints.nirt.res.in/id/eprint/1086

Actions (login required)

View Item