Bioprospecting of marine derived actinomycetes with special reference to antimycobacterial activity

Radhakrishnan, M and Balagurunathan, R and Selvakumar, N and Doble, M and Kumar, Vanaja (2011) Bioprospecting of marine derived actinomycetes with special reference to antimycobacterial activity. Indian Journal of Geo-Marine Science, 40 (3). pp. 407-410. ISSN Online: 2582-6727 | Print: 2582-6506

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Abstract

Present study consists the bioprospecting of marine actinomycetes with special reference to antimycobacterial activity. Totally 49 actinomycetes were isolated from sediment samples collected from mangrove area of Pitchavaram coastal areas of Parangipettai and Andaman & Nicobar Islands and by using starch casein agar medium. Based on the cultural and microscopic characteristics, 30 isolates were identified as Streptomyces sp. and 19 as rare actinomycetes including 11 Micromonospora sp. Bioactive substance from all the isolates were produced by shake flask fermentation method using yeast extract malt extract broth. Culture filtrate and mycelial methanol extracts were tested against drug sensitive and drug resistant clinical isolates of M. tuberculosis and standard strain Mycobacterium tuberculosis H37Rv by luciferase reporter phage (LRP) assay. Culture filtrate and/or mycelial methanol extracts from 41 out of 49 actinomycetes inhibited at least one of the M. tuberculosis strains tested. Culture filtrates from actinomycetes strains viz., R6 ( Streptomyces sp), M1A6 (Micromonospora sp), M1A15 ( Actinosynnemma sp), M1A18 ( Micromonospora sp) and M1A23 (rare actinomycete) showed more than 90% reduction in RLU by LRP assay. Isolation of active compounds from these potent strains probably would lead to the discovery of novel antiTB drugs.

Affiliation:	ICMR-National Institute for Research in Tuberculosis
Item Type:	Article
Uncontrolled Keywords:	Marine, actinomycetes, antimicobacterial activity, LPR assay
Subjects:	Tuberculosis > Laboratory Research > Bacteriological
Divisions:	Basic Science Research > Bacteriology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	17 Jun 2022 09:52
Last Modified:	17 Jun 2022 09:52
URI:	http://eprints.nirt.res.in/id/eprint/1096

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