Simple and rapid liquid chromatography method for determination of moxifloxacin in saliva

Hemanth Kumar, A K and Sudha, V and Srinivasan, R and Ramachandran, Geetha (2011) Simple and rapid liquid chromatography method for determination of moxifloxacin in saliva. Journal of Chromatography B, 879 (30). pp. 3663-3667. ISSN 1570-0232

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Abstract

A high performance liquid chromatographic method for determination of moxifloxacin in human saliva was developed. The method involved deproteinisation of the sample with perchloric acid and analysis of the supernatant using a reversed-phase C18 column (150 mm) and fluorescence detection at an excitation wavelength of 290 nm and an emission wavelength of 460 nm. The assay was specific for moxifloxacin and linear from 0.25 to 10.0 �g/ml. The relative standard deviation of intra- and inter-day assays was lower than 10%. The average recovery of moxifloxacin from saliva was 101%. Due to its simplicity, the assay can be used for pharmacokinetic studies of moxifloxacin.

Affiliation:	ICMR-National Institute for Research in Tuberculosis
Item Type:	Article
Uncontrolled Keywords:	Moxifloxacin Saliva HPLC
Subjects:	Tuberculosis > Laboratory Research > Biochemical
Divisions:	Basic Science Research > Biochemistry
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	22 Jun 2022 07:20
Last Modified:	07 Feb 2023 09:53
URI:	http://eprints.nirt.res.in/id/eprint/1125

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