Increased levels of serum granulocyte-macrophage colony-stimulating factor is sssociated with activated peripheral dendritic cells in Type 2 diabetes subjects (CURES-99)

Surendar, J and Mohan, V and Pavankumar, N and Babu, Subash (2012) Increased levels of serum granulocyte-macrophage colony-stimulating factor is sssociated with activated peripheral dendritic cells in Type 2 diabetes subjects (CURES-99). Diabetes Technology and Therapeutics, 14 (4). pp. 344-349. ISSN Print: 1520-9156 | Electronic: 1557-8593

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Abstract

Background: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pro-inflammatory cytokine with growth factor–like properties for monocytes and dendritic cells (DCs). In the present study, serum GM-CSF levels and the activation status of DCs were studied in type 2 diabetes mellitus (T2DM) subjects. Methods: Study subjects were recruited from the Chennai Urban Rural Epidemiology Study. Healthy controls (n = 45) and T2DM patients (n = 45) were included in the study. Serum levels of GM-CSF, interleukin-1b, interleukin- 6, and tumor necrosis factor-a were measured. Enumeration of circulating DCs (myeloid [m] and plasmocytoid [p]) and its surface antigen expression were quantified by flow cytometry. Results: The serum GM-CSF levels were significantly higher among diabetes subjects compared with subjects without diabetes and showed a positive correlation with glycated hemoglobin (r = 0.208, P = 0.018). The serum GM-CSF levels were lower in subjects on combined insulin and oral hypoglycemic agents (OHA) treatment (1.09 pg/mL) compared with those taking OHA alone (1.9 pg/mL). The increased GM-CSF levels were associated with the activated phenotype of mDCs and pDCs, as determined by up-regulation of the lineage markers. Conclusion: The activated state of mDCs and pDCs seen among diabetes subjects might be due to the increased levels of GM-CSF and other pro-inflammatory cytokines.

Affiliation:	ICMR-National Institute for Research in Tuberculosis
Item Type:	Article
Uncontrolled Keywords:	Serum Granulocyte-Macrophage, Colony-Stimulating Factor, Peripheral Dendritic Cells, Type 2 Diabetes, CURES-99
Subjects:	Tuberculosis > Laboratory Research > Immunological
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	01 Sep 2022 10:10
Last Modified:	01 Sep 2022 10:10
URI:	http://eprints.nirt.res.in/id/eprint/1150

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