A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-1 in sera from leprosy patients

Kumar, S and Moudgil, K D and Band, A H and Narayanan, P R and Gupta, S K and Sharma, A K and Talwar, G P (1986) A dot enzyme immunoassay for detection of IgM antibodies against phenolic glycolipid-1 in sera from leprosy patients. Indian Journal of Leprosy, 58 (2). pp. 185-190. ISSN 0024-1024

Preview

PDF 198601.pdf - Published Version Download (318kB)

Abstract

A visual dipstick dot enzyme immunoassay (EIA) for diagnosis of leprosy is described. The assay is based on detection of IgM antibodies against phenolic glycolipid (PGL-I) in sera from leprosy patients. The antigen (PGL-1 or synthetic disaccharide of PGL-I) was dotted on a nitrocellulose pad stuck on a plastic strip (dipstick). Sera were used at a dilution of 1:200. Peroxidase coupled mouse anti-human IgM monoclonal antibodies were used as the conjugate. A positive test gave a blue dot against a white background. The test was highly specific for leprosy, and was quite sensitive for detection of bacilliferous (BL/LL) leprosy. The antigen dotted and preblocked dipsticks stored at room temperature upto 4 months of observation period, were unable in the assay.

Item Type:	Article
Subjects:	Tuberculosis > Clinical Research
Divisions:	Clinical Research
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	13 Aug 2013 09:17
Last Modified:	17 Mar 2016 08:51
URI:	http://eprints.nirt.res.in/id/eprint/200

Actions (login required)

View Item