Selective up-regulation of endothelial cell class I MHC expression by cytokines from patients with lymphatic filariasis

Freedman, D O and Nutman, T B and Jamal, S and Kumaraswami, V and Ottesen, E A (1989) Selective up-regulation of endothelial cell class I MHC expression by cytokines from patients with lymphatic filariasis. Journal of Immunology, 142 (2). pp. 653-656. ISSN Print: 0022-1767; Online: 1550-6606

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Abstract

Local host immune responses to the lymphaticdwelling filarial parasite Wuchereria bancrofti are important in the pathogenesis of the lymphangitis that leads to filarial elephantiasis. That the lymphatic endothelial cells may be important in this inflammatory process was shown by the ability of supernatants generated from filarial Ag-driven PBMC of individuals with filarial elephantiasis caused by W. brancrofti infection to up-regulate class I MHC expression on human umbilical vein endothelial cells when compared to unstimulated control supernatants from the same individual (relative fluorescence intensity = 159% ± 13.5: p < 0.001). In contrast, individuals with the same filarial infection but manifesting no lymphatic disease were unable to generate, in response to filarial Ag the cytokines required for this activation (relative fluorescence intensity = 93% ± 2.6). Supernatants induced by a non-filarial Ag (purified protein derivative) were able to effect class I MHC upregulation in both patient groups. The same filarial Ag-driven supernatants did not cause detectable class II MHC staining on human umbilical vein endothelial cells. These results suggest a likely role for parasite Agdriven, cytokine-mediated endothelial cell activation in the pathogenesis of lymphatic inflammatory/ obstructive filarial disease.

Item Type:	Article
Subjects:	Tuberculosis > Clinical Research Filariasis
Divisions:	Clinical Research
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	26 Aug 2013 09:10
Last Modified:	14 Mar 2016 10:09
URI:	http://eprints.nirt.res.in/id/eprint/252

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