Observations on the cultivation of M.leprae and M. tuberculosis in medium V and V1

Herbert, D and Prabhakar, R (1992) Observations on the cultivation of M.leprae and M. tuberculosis in medium V and V1. Indian Journal of Leprosy, 64 (3). pp. 341-347. ISSN 0024-1024

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Abstract

Skin scrapings from five different active sites were collected from 14 leprosy patients and inoculated into medium V. Shin scrapings from three leprosy patients were inoculated into medium V 1. All the cultures were incubated at 8-10°C. M. tuberculosis H37Rv, pretreatment isoloates and streptomycin resistant strains were inoculated into medium V, with and without antibiotics, and incubated at 8-10°C as well as 37°C. Smears were made from the M. leprae and M. tuberculosis cultures at 0 hours and at different time points. The number of bacilli in the smears were counted. There was no increase in the number of M. leprae or M. tuberculosis in any of the cultures.

Item Type:	Article
Subjects:	Tuberculosis > Laboratory Research > Bacteriological
Divisions:	Basic Science Research > Bacteriology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	10 Sep 2013 10:14
Last Modified:	14 Mar 2016 07:22
URI:	http://eprints.nirt.res.in/id/eprint/314

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