Restriction fgragment length polymorphism typing of clinical isolates of mycobacterium tuberculosis from patients with pulmonary tuberculosis in Madras, India, by use of direct-repeat probe

Sahadevan, R and Narayanan, S and Paramasivan, C N and Prabhakar, R and Narayanan, P R (1995) Restriction fgragment length polymorphism typing of clinical isolates of mycobacterium tuberculosis from patients with pulmonary tuberculosis in Madras, India, by use of direct-repeat probe. Journal of Clinical Microbiology, 33 (11). pp. 3037-3039. ISSN Print: 0095-1137; Online: 1098-660X

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Abstract

Large numbers of Mycobacterium tuberculosis isolates that were obtained from patients’ sputa on diagnosis and during follow-up after short-course chemotherapy in Madras, India. have either no copy or only a single copy of IS6110. This poses a limitation for DNA fingerprinting with an IS6110-based probe to determine the frequency of exogenous reinfection versus that of endogenous reactivation. In the present study, we overcame this limitation by using an alternate probe, the direct-repeat element. Comparison of pre- and posttreatment isolates by direct-repeat restriction fragment length polymorphism analysis indicated a high degree of endogenous reactivation among patients who have relapses after the successful completion of chemotherapy.

Item Type:	Article
Subjects:	Tuberculosis > Laboratory Research > Immunological
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	26 Sep 2013 10:45
Last Modified:	14 Mar 2016 05:29
URI:	http://eprints.nirt.res.in/id/eprint/400

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