Effect of p-aminosalicylic acid (PAS) on the loss of acid-fastness produced in tubercle bacilli by isoniazid

Varma, K G (1961) Effect of p-aminosalicylic acid (PAS) on the loss of acid-fastness produced in tubercle bacilli by isoniazid. Indian Journal of Medical Research, 49 (2). pp. 194-195. ISSN 0971-5916

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Abstract

The microbiological assay method for estimating free isoniazid in serum described by Mandel et al. (1956), used as the assay end-point the dilution pro-ducing loss of acidfastness in 50 per cent of bacilli. The advantage of this end point is that loss of acidfastness is produced specifically by isoniazid, and not by either PAS (Mandel et al., loc. cit.) or streptomycin (Middlebrook, 1952). Mandel et al. (loc. cit.) claimed that the addition of as much as 1,000 mg./c.c. of PAS to undiluted human sera did not interfere with the assay of isoniazid in the serum using loss of acid-fastness as the end-point. Since Mandel et al. (loc. cit.) did not however, describe their results fully, it remained a possibility that PAS in con-centrations near that necessary to inhibit the growth of tubercle bacilli c ould act synergestically or antagonistically with isoniazid in producing loss of acid-fastness. This was investigated in the following experiments.

Item Type: Article
Subjects: Tuberculosis > Laboratory Research
Divisions: Basic Science Research
Depositing User: Rathinasabapati R
Date Deposited: 16 Jul 2013 05:40
Last Modified: 08 Mar 2016 04:34
URI: http://eprints.nirt.res.in/id/eprint/41

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