Restriction fragment length polymorphism of Mycobacterium tuberculosis strains from various regions of India, using direct repeat probe

Narayanan, S and Sahadevan, R and Narayanan, P R and Krishnamurthy, P V and Paramasivan, C N and Prabhakar, R (1997) Restriction fragment length polymorphism of Mycobacterium tuberculosis strains from various regions of India, using direct repeat probe. Indian Journal of Medical Research, 106 (Nov). pp. 447-454. ISSN 0971-5916

Preview

PDF 199725.pdf - Published Version Download (370kB)

Abstract

Intraspecies differentiation was studied on 68 M. tuberculosis strains obtained from 6 states of India by restriction fragment length polymorphism (RFLP) using a direct repeat probe (DR probe) hybridised with Alu I digest of DNA. Most strains showed polymorphism based patterns that comprised between 2 to 7 bands and were grouped into 26 RFLP types. Of the 11 strains tested from Amritsar, 8 were RFLP type 5; the remaining 3 were of type 11 and were exclusively confined to this region. The strains from other regions were more heterogeneous. We confirm that DR-associated RFLP can be an excellent tool for the differentiation of M. tuberculosis strains. Depending on their geographical origin, these strains can be differentiated to a large extent by DR fingerprinting.

Item Type:	Article
Subjects:	Tuberculosis > Laboratory Research > Immunological
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	29 Oct 2013 09:55
Last Modified:	14 Mar 2016 04:43
URI:	http://eprints.nirt.res.in/id/eprint/450

Actions (login required)

View Item