Bioluminescence assay of adenosine triphosphate in drug susceptibility testing of Mycobacterium tuberculosis

Kumar, V and Selvakumar, N and Venkatesan, P and Chandrasekaran, V and Paramasivan, C N and Prabhakar, R (1998) Bioluminescence assay of adenosine triphosphate in drug susceptibility testing of Mycobacterium tuberculosis. Indian Journal of Medical Research, 107 (Feb). pp. 75-77. ISSN 0971-5916

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Abstract

Twenty three clinical isolates of M. tuberculosis and the reference strain, M. tuberculosis H37Rv were tested for their susceptibility to trifluoperazine (TFP) by the standard broth dilution method and the bioluminescence assay. The results showed that in 15 of the 23 isolates, the minimal inhibitory concentration (MIC) was identical in both the methods and in the remaining 8 isolates the difference in the MIC values between the methods, was less than two fold and was not significant. The findings suggest that the measurement of adenosine triphosphate (ATP) by bioluminescence assay can be employed as an alternative method for the rapid screening of clinical isolates for their susceptibility to anti-mycobacterial agents.

Item Type:	Article
Uncontrolled Keywords:	ATP; bioluminescence assay; Mycobacterium tuberculosis; susceptibility test
Subjects:	Tuberculosis > Laboratory Research > Bacteriological
Divisions:	Basic Science Research > Bacteriology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	30 Oct 2013 08:33
Last Modified:	14 Mar 2016 04:24
URI:	http://eprints.nirt.res.in/id/eprint/482

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