Serologic response to a secreted and a cytosolic antigen of Mycobacterium tuberculosis in childhood tuberculosis

Raja, Alamelu and Ranganathan, Uma Devi and Bethunaickan, Ramalingam and Dharmalingam, Vijayasekaran (2001) Serologic response to a secreted and a cytosolic antigen of Mycobacterium tuberculosis in childhood tuberculosis. Pediatric Infectious Disease Journal, 20 (12). pp. 1161-1164. ISSN Print 0891-3668; Online 1532-0987

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Abstract

Background and aim. Bacteriologic diagnosis of childhood tuberculosis is difficult, and alternate methods are needed. The utility of a serologic test for major secretory antigen (30 kDa) and a cytosolic antigen (16 kDa) of Mycobacterium tuberculosis was evaluated for the diagnosis of tuberculosis in children. Methods. Enzyme-linked immunosorbent assay was used. Specific IgG, IgA and IgM antibodies were measured in the sera from 26 clinically and/or bacteriologically diagnosed cases of childhood tuberculosis and 61 normal children. Results. Anti-IgG antibodies alone, against both 30- and 16-kDa antigens, were detected in 65.4% of patients. However, by combination of all three isotypes, increased sensitivities of 84.6 and 73%, with a specificity of 96.7% each, were obtained for 30- and 16-kDa antigens, respectively. Conclusions. We found good specificity and reasonably good sensitivity for detection of antibodies by enzyme-linked immunosorbent assay to 30-kDa antigen alone. The 16-kDa antigen did not perform as well.

Item Type: Article
Subjects: Tuberculosis > Laboratory Research > Immunological
Divisions: Basic Science Research > Immunology
Depositing User: Rathinasabapati R
Date Deposited: 06 Nov 2013 07:51
Last Modified: 10 Mar 2016 07:12
URI: http://eprints.nirt.res.in/id/eprint/526

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