Simple and rapid high-performance liquid chromatography method for the determination of ofloxacin concentrations in plasma and urine

Immanuel, Chandra and Hemanth Kumar, A K (2001) Simple and rapid high-performance liquid chromatography method for the determination of ofloxacin concentrations in plasma and urine. Journal of Chromatography B, 760. pp. 91-95. ISSN 1570-0232

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Abstract

A high-performance liquid chromatographic method for the determination of ofloxacin in human plasma and urine was developed. The method involved deproteinisation of the sample with perchloric acid and analysis of the supernatant using a reversed-phase C column and fluorescence detection at an excitation wavelength of 290 nm and an emission wavelength of 18 460 nm. The assay was linear from 0.5 to 10.0 mg/ ml. The relative standard deviation of intra- and inter-day assays was lower than 5%. The average recovery of ofloxacin from plasma was 93%. The method was evaluated in samples from healthy subjects whose drug levels were already measured by microbiological assay. Ó 2001 Elsevier Science B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Ofloxacin
Subjects: Tuberculosis > Laboratory Research > Biochemical,Clinical and Pharmacological
Divisions: Basic Science Research > Biochemistry and Pharmacology
Depositing User: Rathinasabapati R
Date Deposited: 06 Nov 2013 10:51
Last Modified: 10 Mar 2016 07:05
URI: http://eprints.nirt.res.in/id/eprint/529

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