Reduced NK activity in pulmonary tuberculosis patients with/without HIV infection: identifying the defective stage and studying the effect of interleukins on NK activity

Nirmala, R and Narayanan, P R and Mathew, R and Maran, M and Deivanayagam, C N (2001) Reduced NK activity in pulmonary tuberculosis patients with/without HIV infection: identifying the defective stage and studying the effect of interleukins on NK activity. Tuberculosis, 81 (5-6). pp. 343-352. ISSN 1472-9792

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Abstract

Setting:A study was undertaken to understand the non-major histocompatibility restricted cytotoxicity in order to delineate the role of natural killer (NK) cells towards the development of host immunity to tuberculosis. Objective: (a) Enumeration of NK cellnumbers and activity in normal individuals (35), pulmonary tuberculosis patients (32),HIV-infected TB patients (20) and patient contacts (10), (b) effect of treatment on NK status, (c) enumeration of effector- target conjugates and (d) effect of in vitro cytokine stimulation on NKactivity. Design:NK cellswere enumerated by flowcytometry.NKactivity was assessed by chromiumrelease assay before and after treatment for tuberculosisandafter stimulationwith IL-2/IL-12.Novel flowcytometricmethodwasstandardizedto enumerate effector-target conjugates. Results: No changeswere seen between different groups as faras numberof NK cells and relative proportions of different conjugate typeswere concerned, but therewas a decrease in NKactivity in TB patientswhich increased after treatment.Augmentation of NKactivity was observed after cytokine stimulation. Conclusion: Lowered NKactivity during tuberculosisinfection isprobably the`effect'and not the`cause' for the disease as demonstrated by the follow-up study. Similar numberof conjugates in both groups indicates no defect in the recognition/ binding step but probably at subsequent steps of the cytotoxic process. Augmentation of NK activity with cytokines implicates themas potential adjuncts to tuberculosis chemotherapy

Item Type:	Article
Subjects:	Tuberculosis > Laboratory Research > Immunological
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	07 Nov 2013 05:46
Last Modified:	10 Mar 2016 07:07
URI:	http://eprints.nirt.res.in/id/eprint/534

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