A recombinant antigen-based lgG4 ELSA for the specific and sensitive detection of Brugia malayi infection

Rahmah , N and Lim, B H and Khairul Anuar, A and Shenoy, R K and Kumaraswami, V and Lokman Hakim, S and Chotechuang, P and Kanjanopas, K and Ramachandran, C P (2001) A recombinant antigen-based lgG4 ELSA for the specific and sensitive detection of Brugia malayi infection. Transactions of the Royal Society of Tropical Medicine and Hygiene, 95 (3). pp. 280-284. ISSN Print: 0035-9203; Online:1878-3503

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Official URL: http://trstmh.oxfordjournals.org/content/95/3/280....

Abstract

An IgG4 ELISA based on a novel recombinant antigen was evaluated for detection of Brugia malayi infection, using 2487 sera from various institutions: 2031 samples from Universiti Sains Malaysia, 276 blinded sera from 2 other institutions in Malaysia, 140 blinded sera from India and 40 blinded sera from Thailand. These sera were from various groups of individuals, i.e., microfilaraemics, chronic patients, endemic normals, non-endemic normals and individuals with other parasitic and bacterial infections. Based on a cut-off optical density reading of 0.300, the IgG4 ELISA demonstrated specificity rates of 95.6- l00%, sensitivity rates of 96- l00%, positive predictive values of 75- 100% and negative predictive values of 98.9- 100%. These evaluation studies demonstrated the high specificity and sensitivity of this test for the detection of active B. malayi infection. Thus, the IgG4 ELISA would be very useful as a tool in diagnosis and in elimination programmes for brugian filariasis.

Item Type: Article
Uncontrolled Keywords: filariasis; Brugia malayi; diagnosis; IgG4; recombinant antigen; ELISA; evaluation; Malaysia; India; Thailand
Subjects: Tuberculosis > Laboratory Research > Bacteriological
Filariasis
Divisions: Basic Science Research > Bacteriology
Depositing User: Rathinasabapati R
Date Deposited: 07 Nov 2013 09:01
Last Modified: 10 Mar 2016 07:11
URI: http://eprints.nirt.res.in/id/eprint/546

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