Washing of new microscopic glass slides in dichromate solution does not influence sputum AFB smear results

Selvakumar, N and Prabhakaran, E and Rehman, F and Frieden, T R and Santha, T (2002) Washing of new microscopic glass slides in dichromate solution does not influence sputum AFB smear results. International Journal of Tuberculosis and Lung Disease, 6 (3). pp. 270-272. ISSN Print: 1027-3719; Online: 1815-7920

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Abstract

SETTING: Microscopy centres in Tiruvallur District, Tamil Nadu, India, implementing DOTS. OBJECTIVE: To know whether washing new glass slides in dichromate solution is essential for effective sputum acid-fast bacilli microscopy. METHODS: Two direct smears were prepared from each of 1750 sputum samples. One was made on dichromate solution-cleaned new glass slides and the other was made on unwashed new glass slides. The smears were blinded and examined. RESULTS: Of the 1750 specimens, 13.5% and 13.08% were positive for AFB using washed and unwashed slides, respectively (P 5 0.12). The concordance between these two (including one grade above and one below) was 98.7%. CONCLUSION: Washing of new glass slides in dichromate solution is not essential for AFB microscopy.

Item Type:	Article
Uncontrolled Keywords:	tuberculosis; sputum; AFB; DOTS; slides
Subjects:	Tuberculosis > Laboratory Research > Bacteriological
Divisions:	Basic Science Research > Bacteriology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	08 Nov 2013 10:53
Last Modified:	10 Mar 2016 04:44
URI:	http://eprints.nirt.res.in/id/eprint/576

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