Purification and Characterization of Three Immunodominant Proteins (38, 30, and 16 kDa) of Mycobacterium tuberculosis

Uma Devi, K R and Senthil Kumar, K S and Ramalingam, B and Alamelu, Raja (2002) Purification and Characterization of Three Immunodominant Proteins (38, 30, and 16 kDa) of Mycobacterium tuberculosis. Protein Expression and Purification , 24 (2). pp. 188-195. ISSN Print: 1046-5928; Online: 1096-027

Preview

PDF 200243.pdf - Published Version Download (226kB)

Abstract

Specific mycobacterial antigens are an important prerequisite in the serodiagnosis of tuberculosis. Many studies have reported the use of both native and recombinant proteins. Even though recombinant proteins can form standardized reagents with unlimited supply, their diagnostic test characteristics were not satisfactory in some cases. In this study we have purified the 38-, 30- (antigen 85B), and 16-kDa native antigens of Mycobacterium tuberculosis by procedures with limited number of steps. Starting with the secreted antigens of M. tuberculosis H37Rv, the 38-kDa form was purified by preparative isoelectric focusing, followed by preparative electrophoresis.

Item Type:	Article
Subjects:	Tuberculosis > Laboratory Research > Immunological
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	12 Nov 2013 06:53
Last Modified:	10 Mar 2016 04:52
URI:	http://eprints.nirt.res.in/id/eprint/591

Actions (login required)

View Item