Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid

Gurumurthy, Prema and Gayathri, T N and Bhagavathy, S and Venkatesan, P (2004) Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid. Indian Journal of Experimental Biology, 42 (1). pp. 68-73. ISSN Print: 0019-5189; Online 0975-1009

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Abstract

A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent. followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400 μg/ml. The relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine quantitatively and stable for a period of atleast one week in urine stored at-20°C.

Item Type:	Article
Uncontrolled Keywords:	Ethambutol, Amberlite CG-50, Rifampicin, Isoniazid. Pyrazinamide
Subjects:	Tuberculosis > Laboratory Research > Biochemical
Divisions:	Basic Science Research > Biochemistry
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	20 Nov 2013 10:49
Last Modified:	09 Mar 2016 10:14
URI:	http://eprints.nirt.res.in/id/eprint/657

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