Comparison of variants of carbol-fuchsin solution in Ziehl-Neelsen for detection of acid-fast bacilli

Selvakumar, N and Gomathi Sekar, M and Rahman, F and Syamsunder, A and Duraipandian, M and Wares, F and Narayanan, P R (2005) Comparison of variants of carbol-fuchsin solution in Ziehl-Neelsen for detection of acid-fast bacilli. International Journal of Tuberculosis and Lung Disease, 9 (2). pp. 226-229. ISSN Print: 1027-3719; Online: 1815-7920

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Abstract

To evaluate Ziehl-Neelsen (ZN) staining using variants of carbol-fuchsin solution, duplicate smears from 416 samples were stained with ZN, one set with 1% basic fuchsin and the other 0.3%. Another set of duplicate smears from 398 samples were stained with ZN, one with 1% basic fuchsin and the other 0.1%. The coded smears were read and discrepancies resolved. All samples underwent mycobacterial culture. The sensitivity of ZN using 0.3% (65%) and 1% basic fuchsin (62%) was comparable, while it was reduced using 0.1% (74%) compared to 1% basic fuchsin (83%). Reducing the concentration of basic fuchsin below 0.3% in ZN staining was found to significantly reduce its sensitivity.

Item Type:	Article
Uncontrolled Keywords:	pulmonary tuberculosis; Ziehl-Neelsen staining; carbol-fuchsin; AFB
Subjects:	Tuberculosis > Laboratory Research > Bacteriological
Divisions:	Basic Science Research > Bacteriology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	28 Nov 2013 11:07
Last Modified:	09 Mar 2016 08:50
URI:	http://eprints.nirt.res.in/id/eprint/719

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