Aravindhan, V and Narayanan, Sujatha and Gautham, N and Prasad, V and Kannan, P and Jacobs, W R and Narayanan, P R (2006) T-h-2 immunityandCD31CD45RBlow-activatedT cells inmice immunizedwith recombinant bacillusCalmette^Gue¤ rin expressing HIV-1principal neutralizing determinant epitope. FEMS Immunology and Medical Microbiology, 47 (1). pp. 45-55. ISSN Print: 0928-8244l; Online: 1574-695X
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Abstract
The genetic engineering of Mycobacterium bovis–bacillus Calmette–Gue´rin to express foreign epitopes is an attractive strategy in the field of epitope vaccines. We constructed an ‘epitope-trap vector’ with Mycobacterium tuberculosis chaperonin- 10 as a carrier antigen and used it to express the HIV-1 principal neutralizing determinant epitope. We also identified a new chaperonin-10 promoter that was hyperexpressive compared with the heat shock protein-65 promoter. Splenocytes from recombinant bacillus Calmette–Gue´rin-immunized mice showed enhanced lymphocyte proliferation and interleukin-4 (but not interferon-g) secretion. The recombinant bacillus Calmette–Gue´rin-immunized group also exhibited mild delayed-type hypersensitivity reaction and a high frequency of CD31CD45RBlow- activated T cells, together with high titer of antiprincipal neutralizing determinant immunoglobulin G antibodies. Thus, this epitope delivery system induced strong epitope-specific T-h-2 polarization.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | recombinant bacillus Calmette–Gue´ rin (r-BCG); epitope-trap vector; chaperonin-10 (Cpn10) promoter; HIV-1 principal neutralizing determinant (PND); cryptic epitopes |
| Subjects: | Tuberculosis > Laboratory Research > Immunological |
| Divisions: | Basic Science Research > Immunology |
| Depositing User: | Dr. Rathinasabapati R |
| Date Deposited: | 02 Dec 2013 09:20 |
| Last Modified: | 31 Dec 2015 07:22 |
| URI: | http://eprints.nirt.res.in/id/eprint/739 |
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