Simple and rapid liquid chromatography method for determination of efavirenz in plasma

Ramachandran, Geetha and Hemanth Kumar, A K and Swaminathan, Soumya and Venkatesan, P and Kumaraswami, V and Greenblatt, D J (2006) Simple and rapid liquid chromatography method for determination of efavirenz in plasma. Journal of Chromatography B, 835 (1-2). pp. 131-135. ISSN 1570-0232

PDF 200611.pdf - Published Version Restricted to Registered users only Download (210kB) | Request a copy

Abstract

A simple and rapid high performance liquid chromatographic method for determination of efavirenz in human plasma was developed. The method involved extraction of sample with ethyl acetate and analysis using a reversed-phase C18 column (150 mm) with UV detection. The assay was linear from 0.0625 to 10.0 �g/ml. The method was specific for efavirenz estimation and the drug was stable in plasma up to one month at −20 ◦C. The average recovery of efavirenz from plasma was 101%. Due to its simplicity, the assay can be used for pharmacokinetic studies and therapeutic drug monitoring of efavirenz.

Item Type:	Article
Uncontrolled Keywords:	Efavirenz; Plasma; HPLC
Subjects:	Tuberculosis > Laboratory Research > Biochemical
Divisions:	Basic Science Research > Biochemistry
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	03 Dec 2013 11:17
Last Modified:	09 Mar 2016 06:20
URI:	http://eprints.nirt.res.in/id/eprint/744

Actions (login required)

View Item