Effect of 1,25 dihydroxyvitamin D3 on intracellular IFN-γ (gamma) and TNF-α(alpha) positive T cell subsets in pulmonary tuberculosis

Prabhu Anand, S and Selvaraj, P and Narayanan, P R (2009) Effect of 1,25 dihydroxyvitamin D3 on intracellular IFN-γ (gamma) and TNF-α(alpha) positive T cell subsets in pulmonary tuberculosis. Cytokine, 45 (2). pp. 105-110. ISSN 1043-4666; eISSN: 1096-0023

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Abstract

We studied the immunomodulatory effect of 1,25(OH)2D3 on single cell expression of IFN-c and TNF-a cytokines in T cell subsets of pulmonary tuberculosis (PTB) patients (n = 22) and normal healthy subjects (n = 22). Peripheral blood mononuclear cells (PBMCs) were cultured with live Mycobacterium tuberculosis (MTB) with or without 1,25(OH)2D3 (10�7 M) for 48 h. T cell subsets positive for IFN-c and TNF-a were enumerated by flow cytometry and the culture supernatants were assayed for both the cytokines using ELISA. In both NHS and PTB patients, a significantly reduced percentage of IFN-c and TNF-a expressing CD3+, CD3+CD4+ and CD3+CD8+ T cells were observed in cultures stimulated with live MTB and treated with 1,25(OH)2D3 compared to cultures without 1,25(OH)2D3 (NHS; CD3+ IFN-c+: p < 0.0001; CD3+TNF-a +: p = 0.0292 and PTB; CD3+ IFN-c+: p = 0.0292; CD3+ TNF-a +: p = 0.0028). The levels of IFN-c and TNF-a in the culture supernatants of 1,25(OH)2D3 treated cultures were also found to be significantly decreased in both groups (NHS; IFN-c: p = 0.0001; TNF-a: p < 0.0001) and (PTB; IFN-c: p < 0.0001; TNF-a: p < 0.0001). A positive correlation was observed between IFN-c and TNF-a expressing CD3+CD8+ T cells in MTB stimulated cultures treated with or without 1,25(OH)2D3 in NHS (p = 0.0001; p = 0.001, respectively) and PTB patients (p = 0.002; p = 0.005, respectively). The present study revealed the suppressive effect of 1,25(OH)2D3 on single cell expression of IFN-c and TNF-a by CD3+CD4+ and CD3+CD8+ T cells in pulmonary tuberculosis. This suppressive effect of 1,25(OH)2D3 on proinflammatory and Th1 cytokine positive cells might have a role in reducing inflammation at the site of infection.

Item Type: Article
Uncontrolled Keywords: 1,25 Dihydroxyvitamin D3 Intracellular cytokines IFN-γ (gamma) TNF-α (alpha) Tuberculosis
Subjects: Tuberculosis > Laboratory Research > Immunological
Tuberculosis
Divisions: Basic Science Research > Immunology
Depositing User: Rathinasabapati R
Date Deposited: 03 Aug 2017 09:46
Last Modified: 03 Aug 2017 09:48
URI: http://eprints.nirt.res.in/id/eprint/930

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