MycobacteriumtuberculosisgroE promoter controls the expression of the bicistronicgroESL1 operonand shows di¡erential regulation under stress conditions

Aravindan, V and Christy, A J and Roy, S and Ajitkumar, P and Narayanan, P R and Narayanan, Sujatha (2009) MycobacteriumtuberculosisgroE promoter controls the expression of the bicistronicgroESL1 operonand shows di¡erential regulation under stress conditions. FEMS Microbiology Letters, 292 (1). pp. 42-49. ISSN Print: 0378-1097; Online: 1574-6968

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Abstract

Heat shock promoters of mycobacteria are strong promoters that become rapidly upregulated during macrophage infection and thus serve as valuable candidates for expressing foreign antigens in recombinant BCG vaccine. In the present study, a new heat shock promoter controlling the expression of the groESL1 operon was identified and characterized. Mycobacterium tuberculosis groESL1 operon codes for the immunodominant 10 kDa (Rv3418c, GroES/Cpn10/Hsp10) and 60 kDa (Rv3417c, GroEL1/Cpn60.1/Hsp60) heat shock proteins. The basal promoter region was 115 bp, while enhanced activity was seen only with a 277-bp fragment. No promoter element was seen in the groES–groEL1 intergenic region. This operon codes for a bicistronic mRNA transcript as determined by reverse transcriptase-PCR and Northern blot analysis. Primer extension analysis identified two transcriptional start sites (TSSs) TSS1 (�236) and TSS2 (�171), out of which one (TSS2) was heat inducible. The groE promoter was more active than the groEL2 promoter in Mycobacterium smegmatis. Further, it was found to be differentially regulated under stress conditions, while the groEL2 promoter was constitutive.

Item Type: Article
Uncontrolled Keywords: Mycobacterium tuberculosis ; GroES; GroEL; promoter; transcription and operon
Subjects: Tuberculosis > Laboratory Research
Tuberculosis > Laboratory Research > Immunological
Tuberculosis
Divisions: Basic Science Research > Immunology
Depositing User: Rathinasabapati R
Date Deposited: 04 Aug 2017 10:39
Last Modified: 04 Aug 2017 10:39
URI: http://eprints.nirt.res.in/id/eprint/938

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