Di¡erential upregulation of chemokine receptors onCD561NKcells and their transmigration tothe site of infection in tuberculous pleurisy

Pokkali, Supriya and Das, S D and Selvaraj, Anbalagan (2009) Di¡erential upregulation of chemokine receptors onCD561NKcells and their transmigration tothe site of infection in tuberculous pleurisy. FEMS Immunology and Medical Microbiology (Now Pathogens and Disease), 55 (3). pp. 302-360. ISSN eISSN: 2049-632X

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Abstract

Chemokines and their receptors orchestrate leukocyte recruitment and confer immunity during Mycobacterium tuberculosis infection. The immunoregulatory and cytotoxic activities of natural killer (NK) cells are essential at the site of infection during tuberculous pleurisy. The frequency, subtypes, and expression of phenotype markers and chemokine receptors on NK cells were assessed by flow cytometry in tuberculous (TB) and nontuberculous (NTB) pleural fluid (PF). Chemotaxis was also shown in response to chemokines. A significant decrease in CD56dim with no change in CD56bright NK cells was observed, while a significant increase in activation markers and Toll-like receptors (TLRs) was observed on TBPF CD56bright NK cells. Significantly increased expression of chemokine receptors CCR1, CCR2 and CCR7 on CD56bright and CCR5 on CD56dim NK cells was observed in the TB group. Transmigration of TB-PF NK cells was significantly high in response to IL-8, IP-10, MCP-1 and SLC. Transmigrated TB-NK cells showed a significant increase in CXCR2, CCR2 and CCR7 expression. The study suggests that CD56bright NK cells may recognize M. tuberculosis directly using TLRs, HLADR and express CD69 as an early activation marker. In addition, CC chemokines induce activation signals in chemokine receptors mediating differential NK cell migration to the site. Thus, NK cells act as first direct sensors and effectors in mycobacterial infection.

Item Type:	Article
Additional Information:	DOI: 10.1111/j.1574-695X.2008.00520.x
Uncontrolled Keywords:	tuberculous pleurisy; NK cells; chemokine; chemokine receptor.
Subjects:	Tuberculosis > Laboratory Research > Immunological Tuberculosis
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	09 Aug 2017 11:00
Last Modified:	02 Mar 2021 06:13
URI:	http://eprints.nirt.res.in/id/eprint/942

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