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IgE responses in human filariasis. 1. Quantitation of filaria-specific IgE.

Hussai, Rabia and Hamilton, Robert G and Kumaraswami, V and Franklin Adkinson, N and Ottesen, E A (1981) IgE responses in human filariasis. 1. Quantitation of filaria-specific IgE. Journal of Immunology, 127 (4). pp. 1623-1629. ISSN Print: 0022-1767; Online: 1550-6606

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We have developed a noncompetitive solid phase radioimmunoassay to quantitate human IgE antibodies against soluble adult antigens of Brugia malayi (Em.), a filarial parasite, in sera of patients with various forms of clinical filariasis in Madras, India. A single reference serum was shown to contain 23 pg/ml of 6.m.-specific IgE by depletion analysis and was used as a standard serum throughout the study. The levels of specific IgE ranged in the patients sera from 2 to 23,000 ng/ml. When these individuals were divided into clinical groups, the individuals with tropical pulmonary eosinophilia had the highest levels (mean = 8630 ng/ml) and were significantly higher than all the other groups (p -= 0.001). The lowest levels were seen in patients with circulating microfilariae (mean = 30.5 ng/ ml). Patients exhibiting lymphatic obstruction (i.e., chronic pathology group) had levels slightly higher than microfilaremics (mean = 68 ng/ml) but were not significantly different (p < 0.1). Surprisingly, individuals living in endemic areas but who had no clinical signs of filariasis also showed appreciable levels of B.m.-specific IgE (mean = 55 ng/ml). The B.m.-specific IgE represented 0.1 to 48% of the total IgE. High percentages of specific IgE may be responsible for evoking allergic symptomatology in patients with tropical pulmonary eosinophilia.

Item Type: Article
Subjects: Tuberculosis > Clinical Research
Divisions: Clinical Research
Depositing User: Dr. Rathinasabapati R
Date Deposited: 30 Jul 2013 08:24
Last Modified: 14 Mar 2016 08:54

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