Interferon gamma gene +874A/T polymorphism and intracellular interferon gamma expression in pulmonary tuberculosis

Prabhu Anand, S and Harishankar, M and Selvaraj, P (2010) Interferon gamma gene +874A/T polymorphism and intracellular interferon gamma expression in pulmonary tuberculosis. Cytokine, 49 (2). pp. 130-133. ISSN ISSN: 1043-4666

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Abstract

We investigated whether IFN-c gene +874(A/T) polymorphism influences intracellular interferon gamma expression in T-cell subsets of normal healthy subjects (NHS) and pulmonary tuberculosis patients (PTB). Peripheral blood mononuclear cells were stimulated with live Mycobacterium tuberculosis (MTB) and the intracellular IFN-c expression was studied using flow cytometry. Genotyping of IFN-c gene +874(A/T) was done using allele specific polymerase chain reaction. Significantly increased IFN-c expressing CD3+CD4+ and CD3+CD8+ T cells were observed in NHS with AA genotype compared to TT genotype in unstimulated (p = 0.0308 and p = 0.0157) and MTB stimulated (p = 0.0494 and p = 0.0287) cultures and this difference was not observed in PTB patients. The present study suggests that the variant genotypes of IFN-c (+874) may be associated with altered expression of IF

Affiliation:	National Institute for Research in Tuberculosis
Item Type:	Article
URI:	http://eprints.nirt.res.in/id/eprint/1023

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