Next‑Gen Dual Transcriptomics for Adult Extrapulmonary Tuberculosis Biomarkers and Host–Pathogen Interplay in Human Cells: A Strategic Review

Nesakumar, Manohar and Hanna, Luke Elizabeth and Vetrivel, Umashankar (2023) Next‑Gen Dual Transcriptomics for Adult Extrapulmonary Tuberculosis Biomarkers and Host–Pathogen Interplay in Human Cells: A Strategic Review. Next‑Gen Dual Transcriptomics for Adult Extrapulmonary Tuberculosis Biomarkers and Host–Pathogen Interplay in Human Cells: A Strategic Review.

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Abstract

Tuberculosis (TB) is a major public health concern that results in significant morbidity and mortality, particularly in middle- to low-income countries. Extrapulmonary tuberculosis (EPTB) in adults is a form of TB that affects organs other than the lungs and is challenging to diagnose and treat due to a lack of accurate early diagnostic markers and inadequate knowledge of host immunity. Next-generation sequencing-based approaches have shown potential for identifying diagnostic biomarkers and host immune responses related to EPTB. This strategic review discusses on the significance using primary human cells and cell lines for in vitro transcriptomic studies on common forms of EPTB, such as lymph node TB, brain TB, bone TB, and endometrial TB to derive potential insights. While organoids have shown promise as a model system, primary cell lines still remain a valuable tool for studying host–pathogen interplay due to their conserved immune system, noniPSC origin, and lack of heterogeneity in cell population. This review outlines a basic workflow for researchers interested in performing transcriptomics studies in EPTB, and also discusses the potential of cell-line based dual RNA-Seq technology for deciphering comprehensive transcriptomic signatures, host–pathogen interplay, and biomarkers from the host and Mycobacterium tuberculosis. Thus, emphasizing the implementation of this technique which can significantly contribute to the global anti-TB effort and advance our understanding of EPTB.

Affiliation:	ICMR-National Institute for Research in Tuberculosis
Item Type:	Article
Uncontrolled Keywords:	Transcriptomics · Biomarkers · Dual-RNA sequencing · Extra-pulmonary tuberculosis · Primary human cell cultures · Human cell lines · Tuberculosis
Subjects:	Tuberculosis > Biomedical Informatics Research
Divisions:	Basic Science Research > Virology and Biotechnology
Depositing User:	Mrs. N Lakshmi
Date Deposited:	17 Jan 2024 07:37
Last Modified:	17 Jan 2024 07:37
URI:	http://eprints.nirt.res.in/id/eprint/1984

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