Development of DNA probes for M. tuberculosis

Narayanan, Sujatha and Sahadevan, R and Ramanujam, S and Prabhakar, R and Narayanan, P R (1992) Development of DNA probes for M. tuberculosis. Indian Journal of Tuberculosis, 40 (2). pp. 99-105. ISSN 0019-5705

Preview

PDF 199219.pdf - Published Version Download (689kB)

Abstract

Attempts were made to develop DNA probes for M. tuberculosis. Random library of M. tuberculosis was constructed in plasmid pGEM -4. Selection of recombinant clones was made by hybridisation with 32P labelled M. tuberculosis probe. Ten recombinant clones were selected on the basis of strong signals from the random library. These 10 clones named pTRC1-10 were subjected to tests for specificity and sensitivity. On this basis, pTRC4 was chosen and this is also, useful in restriction fragment length polymorphism (RFLP) studies.

Item Type:	Article
URI:	http://eprints.nirt.res.in/id/eprint/330

Actions (login required)

View Item