Transportation of lymph node biopsy specimens in selective Kirchner’s liquid medium for culture of tubercle bacilli

Vanajakumar, - and Selvakumar, N and Jawahar, M S and Rajaram, K and Paramasivan, C N (1997) Transportation of lymph node biopsy specimens in selective Kirchner’s liquid medium for culture of tubercle bacilli. Journal of Medical Microbiology, 46 (3). pp. 260-262. ISSN Print: 0022-2615; Online: 1473-5644

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Abstract

Lymph node biopsy specimens, obtained from 297 paediatric and adult patients with tuberculous lymphadenitis at Madurai, were transported in selective Kirchner’s liquid medium (KL-T) to the Tuberculosis Research Centre, Madras and processed for culture. Mycobucterium tuberculosis was isolated from 201 (68%) specimens. Of the 192 specimens received within 4 days of resection, 134 (69.8%) yielded M. tuberculosis on culture and of the 105 specimens received after 5 days, 67 (63.8%) were culture positive; the difference was not statistically significant. By incubating KL-T alone further, after removing the gland for processing, it was found that mere contact with the excised node during transportation was enough to retrieve 77 (38.3%) of the total of 201 positive isolates obtained, the delay did not affect the culture positivity rate. Thus, lymph node specimens for culture of tubercle bacilli can be stored in the refrigerator for up to 15 days and transported in KL-T at ambient temperature for 18-20 h without any loss in culture positivity.

Item Type:	Article
Additional Information:	Study Paper
Subjects:	Tuberculosis > Clinical Research
Divisions:	Basic Science Research > Bacteriology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	29 Oct 2013 10:17
Last Modified:	14 Mar 2016 04:57
URI:	http://eprints.nirt.res.in/id/eprint/452

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