Evaluation of PCR Using TRC4 and IS6110 Primers in Detection of Tuberculous Meningitis

Narayanan, Sujatha and Parandaman, Vijayalakshmi and Narayanan, P R and Venkatesan, P and Girish, C and Mahadevan, S and Rajajee, Sarala (2001) Evaluation of PCR Using TRC4 and IS6110 Primers in Detection of Tuberculous Meningitis. Journal of Clinical Microbiology, 39 (5). pp. 2006-2008. ISSN Print: 0095-1137; Online: 1098-660X

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Abstract

We have evaluated a new set of primers (TRC4) in comparison with the IS6110 primers commonly used in PCR to detect tuberculous meningitis among children. The levels of concordance between the results of IS6110 PCR and TRC4 PCR with cerebrospinal fluid specimens from patients with clinically confirmed tuberculous meningitis were 80 and 86%, respectively. Results with the two primer sets were concordant for 55 positive and 22 negative specimens (n 5 98). We conclude that the sensitivity of PCR can be increased by using both IS6110 and TRC4 primers.

Item Type:	Article
Subjects:	Tuberculosis > Laboratory Research > Immunological
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	07 Nov 2013 06:39
Last Modified:	10 Mar 2016 07:07
URI:	http://eprints.nirt.res.in/id/eprint/538

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