Purification of cytochrome P-450 in mycobacteria

Ramachandran, Geetha and Gurumurthy, Prema (2002) Purification of cytochrome P-450 in mycobacteria. Current Science, 83 (7). pp. 873-877. ISSN 0011-3891

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Abstract

Purification of cytochrome P-450 from Mycobacterium smegmatis, M. chelonae, M. fortuitum and M. tuberculosis H37RV was undertaken. The electrophoretic pattern revealed a single band corresponding to a molecular weight of 66 kDa in all the four species. Cytochrome P-450 purified from drug-resistant M. tuberculosis showed a different pattern from that of the sensitive bacteria, and the former was similar to the purified product obtained from phenobarbitalinduced cytochrome P-450 in M. tuberculosis H37RV. It therefore appears that different forms of cytochrome P-450 are present in drug-sensitive and resistant M. tuberculosis, and that there is similarity in the pattern between drug-resistant and phenobarbital-induced M. tuberculosis.

Item Type:	Article
URI:	http://eprints.nirt.res.in/id/eprint/557

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