Evaluation of the Phenol Ammonium Sulfate Sedimentation Smear Microscopy Method for Diagnosis of Pulmonary Tuberculosis

Selvakumar, N and Rahman, Fathima and Garg, R and Rajasekaran, S and Mohan, Nalini Sunder and Thyagarajan, K and Sundaram, V and Santha, T and Frieden, T R and Narayanan, P R (2002) Evaluation of the Phenol Ammonium Sulfate Sedimentation Smear Microscopy Method for Diagnosis of Pulmonary Tuberculosis. International Journal of Tuberculosis and Lung Disease, 40 (8). pp. 3017-3020. ISSN Print: 1027-3719; Online: 1815-7920

Preview

PDF 200230.pdf - Published Version Download (682kB)

Abstract

We compared the sensitivity and specificity of the phenol ammonium sulfate (PhAS) sediment smear microscopy method for detection of acid-fast bacilli with those of direct smear microscopy, using culture results for Mycobacterium tuberculosis as the “gold standard.” The sensitivities of the PhAS and direct smear methods were 85% (465 of 547) and 83% (454 of 547), respectively, and the specificity of each method was 97%. The PhAS method was better accepted by the laboratory technicians and safer but necessitates an overnight sedimentation, which delays reporting of results until 1 day after sputum collection.

Item Type:	Article
Subjects:	Tuberculosis > Laboratory Research > Bacteriological
Divisions:	Basic Science Research > Bacteriology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	08 Nov 2013 11:17
Last Modified:	10 Mar 2016 04:44
URI:	http://eprints.nirt.res.in/id/eprint/578

Actions (login required)

View Item