Evaluation of microplate Alamar blue assay for drug susceptibility testing of Mycobacterium avium complex isolates

Daisy Vanitha, J and Paramasivan, C N (2004) Evaluation of microplate Alamar blue assay for drug susceptibility testing of Mycobacterium avium complex isolates. Diagnostic Microbiology and Infectious Disease, 49 (3). pp. 179-182. ISSN 0732-8893

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Abstract

Fifty-one clinical isolates and 5 clarithromycin-resistant mutants of Mycobacterium avium complex (MAC) were tested for their susceptibility to clarithromycin by microplate Alamar blue assay (MABA). The susceptibility results were compared with the results obtained by the BACTEC 460 method. All clinical isolates were susceptible, while all mutants were resistant to clarithromycin by BACTEC. Eighty-six percent of the clinical isolates were susceptible by MABA, and one of the resistant mutants was misclassified as susceptible by this method. The overall agreement between MABA and BACTEC was 86%, indicating the usefulness of MABA in drug susceptibility testing of MAC.

Item Type:	Article
Subjects:	Tuberculosis > Laboratory Research > Bacteriological
Divisions:	Basic Science Research > Bacteriology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	18 Nov 2013 07:03
Last Modified:	09 Mar 2016 10:06
URI:	http://eprints.nirt.res.in/id/eprint/637

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