Bactericidal Action of Gatifloxacin, Rifampin, and Isoniazid on Logarithmic- and Stationary-Phase Cultures of Mycobacterium tuberculosis

Paramasivan, C N and Sulochana, S and Kubendiran, G and Venkatesan, P and Mitchison, D A (2005) Bactericidal Action of Gatifloxacin, Rifampin, and Isoniazid on Logarithmic- and Stationary-Phase Cultures of Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy, 49 (2). pp. 627-631. ISSN Print: 0066-4804; Online: 1098-6596

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Abstract

The bactericidal activity of gatifloxacin, alone and in combination with isoniazid and rifampin, was studied on both exponential- and stationary-phase cultures of Mycobacterium tuberculosis strain H37Rv. On log-phase cultures, the bactericidal activity of gatifloxacin at 4 �g/ml was rapid and was very similar to that of isoniazid. At concentrations of 0.25 and 4 �g/ml, gatifloxacin enhanced the activity of isoniazid. Killing of the stationaryphase culture was biphasic. During the first 2 days, gatifloxacin at 4 �g/ml slightly increased the limited bactericidal activities of isoniazid and rifampin. However, no further additional bactericidal activity was found during further incubation with isoniazid alone or when gatifloxacin was added to either isoniazid or rifampin. This suggested that the stationary-phase culture contained a mixture of occasionally dividing bacilli that were killed during the first 2 days and true static persisters in the residual population that mimicked those in human lesions. In view of the failure of gatifloxacin to add to the sterilizing activity of isoniazid or rifampin during days 2 to 6 of exposure in the stationary-phase culture, it is unlikely to be a sterilizing drug that can be used to shorten the duration of treatment appreciably when it is added to present treatment regimens.

Item Type:	Article
Subjects:	Tuberculosis > Laboratory Research > Bacteriological
Divisions:	Basic Science Research > Bacteriology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	28 Nov 2013 08:22
Last Modified:	09 Mar 2016 08:39
URI:	http://eprints.nirt.res.in/id/eprint/709

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