Effect of storage for three months at different temperatures on the sensitivity to streptomycin and isoniazid of cultures of tubercle bacilli

Devaki, V and Mohan, K and Gangadharam, P R J (1967) Effect of storage for three months at different temperatures on the sensitivity to streptomycin and isoniazid of cultures of tubercle bacilli. Indian Journal of Medical Research, 55 (11). pp. 1150-1158. ISSN 0971-5916

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Abstract

MAINTENANCE of bacterial strains by repeated subcultivation is both expensive, laborious and time-consuming ; moreover, there is always the possibility of contamination or of differential selection of sub-strains with specific properties. In consequence, several methods, such as freeze-drying or storage at low temperatures, have been introduced by which bacterial cultures can be kept alive for long periods with their reproductive and metabolic activity at an extremely low level. However, information is rather sparse on such methods for the storage of tubercle bacilli. For instance, Corper and Gauss (1923) found that tubercle bacilli remained viable in Petroff's egg medium or glycerol agar after storage in the incubator or refrigerator for 4 to 8 months. Later workers (Heckly, 1950 ; Stern and Tompsett, 1951 ; Jones, 1957 ; Tsukamura, 1965) suggested preservation of cultures by freezing them in various diluents. More recently, Tarshis (1961) compared storage of cultures in various diluents at –20°C. and concluded that, with minor exceptions, most types of mycobacteria (including tubercle bacilli) can be stored for at least 3 years without any major change in their viability or drug resistance. However, these procedures are time-consuming, expensive and require special equipment and are? therefore, not very practicable in developing countries with limited resources.

Item Type:	Article
Subjects:	Tuberculosis > Laboratory Research > Bacteriological
Divisions:	Basic Science Research
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	18 Jul 2013 08:56
Last Modified:	08 Mar 2016 06:16
URI:	http://eprints.nirt.res.in/id/eprint/78

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