1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis

Vidyarani, M and Selvaraj, P and Jawahar, M S and Narayanan, P R (2007) 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis. Cytokine, 40 (2). pp. 128-134. ISSN Print: 1043-4666; Onine: 1096-0023

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Abstract

1, 25 Dihydroxyvitamin D3 (1, 25(OH)2 D3) has gained significant importance in tuberculosis with regard to its immunoregulatory activities. Our aim was to evaluate the effect of 1, 25(OH)2 D3 on cytokine response to Mycobacterium tuberculosis antigens in pulmonary tuberculosis. Peripheral blood mononuclear cells obtained from 60 healthy controls and 52 pulmonary tuberculosis patients were cultured with culture filtrate antigen (CFA) of M. tuberculosis and live M. tuberculosis with and without 1, 25(OH)2 D3 (10�9, 10�8 and 10�7 M concentrations). The culture supernatants were used to estimate IL-8, IL-6, TGF-b, IL-10, IFN-c, IL-12p40, IL-2, IL-4 and IL-5 levels by ELISA. 1, 25 Dihydroxyvitamin D3 significantly suppressed IL-12p40 and IFN-c production in response to CFA and live M. tuberculosis with a maximum suppression at 10�7M concentration (p < 0.0001). In CFA stimulated cultures, addition of 1, 25(OH)2 D3 significantly suppressed IL-8, IL-6 and IL-10 whereas the IL-2 levels were significantly increased in controls. It variably influenced the Th2 cytokines, showing an increased trend for IL-4 and suppressed IL-5 levels. We report that 1, 25(OH)2 D3 differentially modulates production of cytokines in response to M. tuberculosis antigens by predominantly suppressing IL-12p40 and IFN-c production in a dose dependent manner. Our results suggest a role for vitamin D in restricting acquired immune response against tuberculosis by regulating cytokine production.

Item Type:	Article
Uncontrolled Keywords:	Cytokines; M. tuberculosis; 1, 25 Dihydroxyvitamin D3; Tuberculosis
Subjects:	Tuberculosis > Laboratory Research > Immunological
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	10 Jan 2014 09:03
Last Modified:	31 Mar 2021 04:37
URI:	http://eprints.nirt.res.in/id/eprint/839

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