Regulatory Role of Promoter and 3′ UTR Variants of Vitamin D Receptor Gene on Cytokine Response in Pulmonary Tuberculosis

Selvaraj, P and Vidyarani, M and Alagarasu, K and Prabhu Anand, S and Narayanan, P R (2008) Regulatory Role of Promoter and 3′ UTR Variants of Vitamin D Receptor Gene on Cytokine Response in Pulmonary Tuberculosis. Journal of Clinical Immunology, 28 (4). pp. 306-313. ISSN 0271-9142; eISSN: 1573-2592

Archive 200836.pdf - Published Version Restricted to Registered users only Download (315kB) | Request a copy

Abstract

Vitamin D receptor (VDR) gene variants are shown to regulate immune response in tuberculosis. We studied the influence of VDR promoter (Cdx-2 and A1012G), 3′ untranslated region (Apa I, Bsm I, and Taq I) and start codon (Fok I) polymorphisms on 1,25(OH)2D3- modulated IL-12p40, IFN-γ, IL-10, and IL-5 response to live Mycobacterium tuberculosis and its culture filtrate antigen (CFA) in 60 normal healthy subjects and 51 pulmonary tuberculosis patients. In peripheral blood mononuclear cell cultures with CFA and 1,25(OH)2D3, IL-12p40, and IFN-γ levels were significantly decreased (p<0.05) and IL-10 levels were significantly increased (p<0.05) in patients with GG genotype. The extended genotype bbaaTT (baT haplotype) was associated with decreased IL-12p40 and IFN-γ levels and significantly increased IL-10 levels (p<0.05). The Cdx-2 GG genotype and baT haplotype are associated with a suppressed Th1 and increased IL-10 response, which suggests that 1,25(OH)2D3 probably through the VDR polymorphic variants augments the antiinflammatory response at the site of M. tuberculosis infection.

Item Type:	Article
Uncontrolled Keywords:	Vitamin D receptor . cytokines . gene polymorphism . tuberculosis
Subjects:	Tuberculosis > Laboratory Research > Immunological Tuberculosis
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	21 Jul 2017 10:13
Last Modified:	25 Mar 2021 09:33
URI:	http://eprints.nirt.res.in/id/eprint/894

Actions (login required)

View Item