Augumentation of Natural Killer Activity with Exogenous Interleukins in Patients with HIV and Pulmonary Tuberculosis Coinfection

Ramana Rao, P V and Rajasekaran, S and Raja, Alamelu (2008) Augumentation of Natural Killer Activity with Exogenous Interleukins in Patients with HIV and Pulmonary Tuberculosis Coinfection. AIDS Research and Human Retroviruses, 24 (11). pp. 1435-1443. ISSN Print: 0889-2229; Online: 1931-8405

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Abstract

A depressed level of natural killer (NK) activity is one of the various immunological abnormalities in HIV infection. Defective NK cell functions can be partially restored in vitro by interleukin (IL)-2 and IL-12. IL-15 shares receptor and several biological properties with IL-2. The effect of IL-15 on NK cells in patients with HIV and tuberculosis coinfection (HIV-TB) is unclear. This study examined the cytotoxic activity and cytokine response of NK cells in HIV-TB after stimulation with IL-15 and IL-12/IL-2. The study includes 16 normal healthy subjects (NHS), 15 patients with pulmonary tuberculosis (TB), 15 HIV-infected subjects (HIV), and 15 HIV-TB patients. The cytotoxic activity of NK cells was assessed by dioctadecyloxacarbocyanine dye-based flow cytometry. Interferon-γ present in the culture supernatants was measured by enzyme-linked immunosorbent assay. Basal NK cytotoxicity was found to be lower in HIV-TB (p < 0.05) and HIV when compared to NHS or TB. Maximal NK cytotoxicity (p < 0.05) was observed with an IL-15 and IL-12 combination in all the groups. At a 50:1 effector/target ratio, the mean fold increase in NK cytotoxicity upon stimulation was 2.11 for HIV and 1.84 for HIV-TB. Interferon-� levels from the stimulated cultures were elevated (p < 0.05) in the HIV and HIV-TB groups. We found no correlation between NK cytotoxicity and CD4 counts in HIV-TB. There is a positive correlation between NK cytotoxicity and interferon-� secretion for HIV-TB. The combination of IL-15 and IL-12 may have potential to improve the NK activity of HIV and HIV-TB.

Item Type:	Article
Uncontrolled Keywords:	Exogenous, Interleukins, HIV, Pulmonary Tuberculosis Coinfection
Subjects:	Tuberculosis > Laboratory Research > Immunological Tuberculosis
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	31 Jul 2017 10:34
Last Modified:	25 Mar 2021 08:11
URI:	http://eprints.nirt.res.in/id/eprint/915

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