Boosting with a DNA vaccine expressing ESAT-6 (DNAE6) obliterates the protection imparted by recombinant BCG (rBCGE6) against aerosol Mycobacterium tuberculosis infection in guinea pigs

Dey, B and Jain, R and Khera, A and Rao, V and Dhar, N and Gupta, U D and Katoch, V M and Ramanathan, V D and Tyagi, A K (2009) Boosting with a DNA vaccine expressing ESAT-6 (DNAE6) obliterates the protection imparted by recombinant BCG (rBCGE6) against aerosol Mycobacterium tuberculosis infection in guinea pigs. Vaccine, 28 (1). pp. 63-70. ISSN 0264-410X

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Abstract

Owing to its highly immunodominant nature and ability to induce long-lived memory immunity, ESAT-6, a prominent antigen of Mycobacterium tuberculosis, has been employed in several approaches to develop tuberculosis vaccines. Here, for the first time, we combined ESAT-6 based recombinant BCG (rBCG) and DNA vaccine (DNAE6) in a prime boost approach. Interestingly, in spite of inducing an enhanced antigen specific IFN-� response in mice, a DNAE6 booster completely obliterated the protection imparted by rBCG against tuberculosis in guinea pigs. Analysis of immunopathology and cytokine responses suggests involvement of an exaggerated immunity behind the lack of protection imparted by this regimen.

Item Type:	Article
Uncontrolled Keywords:	Tuberculosis; ESAT-6; Prime boost vaccination
Subjects:	Tuberculosis > Laboratory Research > Pathological Tuberculosis
Divisions:	Basic Science Research > Clinical Pathology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	23 Nov 2017 07:07
Last Modified:	02 Mar 2021 05:34
URI:	http://eprints.nirt.res.in/id/eprint/985

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