Raghupati Sarma, G and Immanuel, Chandra and Kailasam, S and Kannapiran, M and Nair, N G K and Radhakrishna, S
(1974)
A Modified Method for the Estimation of Acetylisoniazid in Urine.
Indian Journal of Medical Research, 62 (6).
pp. 954-952.
ISSN 0971-5916
Abstract
The method of Venkataraman et al. (1968) for the direct estimation of acetylisoniazid in
urine has been modified to make it suitable for application to urine specimens containing
sugar. The urine is first extracted with a mixture of chloroform and n-butanol, the
organic phase re-extracted with dilute sulphuric acid, and the original method then
applied to the acid extract. With the modified method, recoveries of acetylisoniazid were
quantitative, both in the presence and the absence of glucose. Interference due to
hydrazones and isonicotinyl glycine was substantially reduced by the modification, while
that due to isoniazid remained the same. There was little or no interference from
isonicotinic acid, with either the original or the modified method.
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