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Phosphocholine - containing antigens of Brugia malayi non-specifically suppress lymphocyte function

Lal, R B and Kumaraswami, V and Steel, Cathy and Nutman, T B (1990) Phosphocholine - containing antigens of Brugia malayi non-specifically suppress lymphocyte function. America Journal of Tropical Medicine and Hygiene , 42 (1). pp. 56-64. ISSN Print: 0002-9637; Online: 1476-1645

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The immunosuppressive effect of Brugia malayi antigen (BmA) on phytohemagglutinin (PHA) driven T cell proliferation was evaluated in patients with filariasis (n = 14) and compared to control individuals (n = 12). When peripheral blood lymphocytes were co-cultured with BmA and PHA, BmA markedly suppressed the T cell proliferative response to PHA in both filarial patients and control individuals in a dose-dependant manner. The suppression resulted neither from any direct toxicity of BmA nor from non-specific absorption of the PHA mitogenic activity by BmA. The major suppressive component appears to be phosphocholine (PC), an immunodominant molecule present in abundance on filarial parasites and on circulating filarial antigen. Both purified PC as well as PC-containing antigens affinity purified from BmA were capable of suppressing the proliferative responses of co-cultured autologous lymphocytes to PHA. The suppressive activity was not abolished by mitomycin-C treatment and was greater in patients with filariasis than in normal controls, suggesting that levels of PC-containing antigens determines the magnitude of the suppressive effect of PC-antigen. Further, as induction of the suppressive activity was completely abrogated when atigen pre-treated cells were T cell-depleted, the suppressive effect appears to be mediated primarily by T cells.

Item Type: Article
Subjects: Tuberculosis > Clinical Research
Divisions: Clinical Research
Depositing User: Dr. Rathinasabapati R
Date Deposited: 30 Aug 2013 10:44
Last Modified: 14 Mar 2016 09:28

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