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Human Tuberculosis Sera Show Prominent Antibody Responses to Particulate Fractions of Mycobacterium tuberculosis

Das, Sulochana and Narayanan, Sujatha and Paramasivan, C N and Lowrie, D B and Narayanan, P R (1991) Human Tuberculosis Sera Show Prominent Antibody Responses to Particulate Fractions of Mycobacterium tuberculosis. Journal of Clinical Immunology, 11 (2). pp. 74-77. ISSN Print: 0271-9142; Online: 1573-2592

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Abstract

Sera from smear-positive pulmonary tuberculosis patients and normal control subjects in Madras were analyzed by Western blotting for their reactivity with soluble and particulate (membrane-rich and cell wall-rich) antigen fractions extracted by sonication from Mycobacterium tuberculosis H37Rv. Discrimination between patient and control sera was best with particulate antigen fractions: 60% of patient sera reacted with a 38-kD antigen band and 90% reacted with a 55-kD band. Reactions of control sera with the 38- and 55-kD bands were infrequent and faint. The results suggest that a serodiagnostic test might be based on quantitation of responses to these two antigens.

NIRT Creators:
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Item Type: Article
Uncontrolled Keywords: Tuberculosis; serology; Western blot; diagnosis.
Subjects: Tuberculosis > Laboratory Research > Immunological
Divisions: Basic Science Research > Immunology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 10 Sep 2013 07:30
Last Modified: 14 Mar 2016 08:34
URI: http://eprints.nirt.res.in/id/eprint/307

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