Fate of Mycobacterium tuberculosis inside rat peritoneal macrophages in vitro

Vishwanath, V and Meera, R and Puvanakrishnan, R and Narayanan, P R (1997) Fate of Mycobacterium tuberculosis inside rat peritoneal macrophages in vitro. Molecular and Cellular Biochemistry, 175. pp. 169-175. ISSN Print: 0300-8177; Online: 1573-4919

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Abstract

Rat peritoneal macrophages in vitro were infected with Mycobacterium tuberculosis and the fate of M. tuberculosis inside macrophages was monitored. Alteration in the levels of nitric oxide (NO) measured in terms of nitrite formed, hydrogen peroxide (H202) and lysosomal enzymes such as acid phosphatase, cathepsin-D and ß-glucuronidase in macrophages following M. tuberculosis infection was also studied. Elevation in the levels of nitrite were observed from 72 h of M. tuberculosis infection. Irrespective of the time point, M. tuberculosis infected macrophages produced elevated levels of H2O2. Maximum increase in the level of acid phosphatase was observed from 72 h of M. tuberculosis infection, whereas maximum elevation in the level of ß-glucuronidase was observed 48 h after M. tuberculosis infection. However these microbicidal agents did not alter the intracellular viability of M. tuberculosis

Item Type:	Article
Subjects:	Tuberculosis > Laboratory Research > Immunological
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	29 Oct 2013 11:15
Last Modified:	14 Mar 2016 05:00
URI:	http://eprints.nirt.res.in/id/eprint/458

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