Narayanan, Sujatha and Vishwanath, V and Narayanan, P R
(2001)
Differential expression of a unique
protein by intracellular
Mycobacterium tuberculosis complex.
Current Science, 81 (6).
pp. 689-692.
ISSN 0011-3891
Abstract
We have investigated the changes in the protein synthesis
pattern in guinea pig peritoneal macrophages
following infection with virulent Mycobacterium tuberculosis
H37Rv in vitro. By 35S methionine labelling
of the newly synthesized proteins followed by
ultracentrifugation, SDS-PAGE (sodium dodecyl
sulphate polyacrylamide gel electrophoresis) and
autoradiography, the protein synthesis pattern of the
control uninfected macrophages and the infected
macrophages in vitro were compared. By adding
cycloheximide to the macrophage cultures, the protein
synthesis of macrophages was inhibited and the
protein synthesis pattern of M. tuberculosis has been
analysed. We have identified a mycobacterial protein
of molecular weight 17 kDa which was expressed
exclusively in the cytosolic fraction of M. tuberculosis-
infected guinea pig macrophages in vitro.
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