Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection

Rahmah , N and Taniawati, S and Shenoy, R K and Lim, B H and Kumaraswami, V and Khairul Anuar, A and Lokman Hakim, S and Noor Hayati, M I and Chan, B T and Suharni, M and Ramachandran, C P (2001) Specificity and sensitivity of a rapid dipstick test (Brugia Rapid) in the detection of Brugia malayi infection. Transactions of the Royal Society of Tropical Medicine and Hygiene, 95 (6). pp. 601-604. ISSN Print: 0035-9203; Online:1878-3503

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A total of 753 serum samples from 6 institutions in 3 countries (Malaysia, Indonesia and India) were used to evaluate an immunochromatographic rapid dipstick test, Brugia Rapid, for diagnosis of Brugia malayi infection. The samples comprised sera from 207 microfilaria-positive individuals and 546 individuals from filaria non-endemic areas. The latter consisted of 70 individuals with soil-transmitted helminth infections, 68 with other helminth infections, 238 with protozoan infections, 12 with bacterial and viral infections and 158 healthy individuals. The dipstick is prepared with a goat anti-mouse antibody control line and a B. malayi recombinant-antigen test line. First, the dipstick is dipped into a well containing diluted patient serum, thus allowing specific anti-filarial antibody in the serum to react with the recombinant antigen. Then the dipstick is placed into an adjacent well containing reconstituted anti-human IgG4-gold. After 10 min, development of 2 red-purplish lines denotes a positive result and one line indicates a negative reaction. The overall results of the evaluation showed 97% sensitivity, 99% specificity, 97% positive predictive value and 99% negative predictive value. Brugia Rapid is thus a promising diagnostic tool for detection of B. malayi infection, and would be especially useful for the brugian filariasis elimination programme.

Item Type: Article
Uncontrolled Keywords: filariasis, Brugia malayi, diagnosis, dipstick, rapid test, recombinant antigen, IgG4, evaluation, Malaysia, Indonesia, India
Subjects: Tuberculosis > Laboratory Research > Bacteriological
Divisions: Basic Science Research > Bacteriology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 07 Nov 2013 09:45
Last Modified: 10 Mar 2016 07:12
URI: http://eprints.nirt.res.in/id/eprint/548

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