Effect of rifampicin and isoniazid on cytochrome P-450 in mycobacteria

Ramachandran, Geetha and Gurumurthy, Prema (2002) Effect of rifampicin and isoniazid on cytochrome P-450 in mycobacteria. Indian Journal of Medical Research, 116 (Oct). pp. 140-144. ISSN 0971-5916

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Background & objectives: Rifampicin and isoniazid are the most important first line drugs used in the treatment of tuberculosis. These drugs are also used in combination with other medications to treat co-infections. It, therefore, becomes important to study the effect of these drugs on the drug metabolizing system, namely, cytochrome P-450, not only in the host but also in the bacteria. We report the effect of rifampicin and isoniazid on the cytochrome P-450 activity in Mycobacterium smegmatis and M tuberculosis H37Rv. Methods: Subinhibitory concentrations of rifampicin and isoniazid were added to the organisms after they had attained the growth phase and cytochrome P-450 activity was estimated in the membranous fractions of the bacteria at different time points. Results: Rifampicin was able to significantly enhance cytochrome P-450 in both M. smegmatis and M. tuberculosis H37Rv. Isoniazid was found to inhibit cytochrome P-450 in M. tuberculosis H37Rv, While there seemed to be no effect in M. smegmatis. Interpretation & conslusion: We report here the effect of rifampicin and isoniazid on mycobacterial cytochrome P-450. These findings are similar to those found in eukaryotic organisms. The role of mycobacterial cytochrome P-450 in the metabolism of drugs within the bacteria needs to be elucidated.

Item Type: Article
Uncontrolled Keywords: Cytochrome P-450, isoniazid, mycobacteria, rifampicin
Subjects: Tuberculosis > Laboratory Research > Biochemical
Divisions: Basic Science Research > Biochemistry
Depositing User: Dr. Rathinasabapati R
Date Deposited: 08 Nov 2013 06:10
Last Modified: 10 Mar 2016 04:41
URI: http://eprints.nirt.res.in/id/eprint/556

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