Senthil Kumar, K S and Uma Devi, K R and Alamelu, Raja
(2002)
Isolation and Evaluation of Diagnostic Value of Two Major
Secreted Proteins of Mycobacterium Tuberculosis.
Indian Journal of Chest Diseases and Allied Sciences, 44 (4).
pp. 225-232.
ISSN 0377 9343
Abstract
Two secreted antigens of Mycobacterium tuberculosis, namely the antigen 85 complex (30/31) and
38kDa antigens, were purified from the whole culture filtrate by using two dimensional
preparative electrophoresis and anion exchange chromatography, respectively. Individual
components of the antigen 85 complex namely, antigen 85A, 85B and 85C, were separated using
hydrophobic interaction chromatography. The humoral antibody activity to these antigens in
sputum positive cases of active pulmonary tuberculosis and normal healthy volunteers was
determined by enzyme linked immunosorbent assay (ELISA) and immunoblot. Recombinant
38kDa and antigen 6 were used as reference antigens for the assay. None of the healthy
volunteers reacted with the 38kDa antigen, while 52% of the TB sera reacted with it. Of the three
components of the antigen 85 complex, 85B gave the highest positivity of 40 per cent. The results
of combination of 38kDa with antigen 6 offered better results with 76% positivity.
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