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Isolation and Evaluation of Diagnostic Value of Two Major Secreted Proteins of Mycobacterium Tuberculosis

Senthil Kumar, K S and Uma Devi, K R and Alamelu, Raja (2002) Isolation and Evaluation of Diagnostic Value of Two Major Secreted Proteins of Mycobacterium Tuberculosis. Indian Journal of Chest Diseases and Allied Sciences, 44 (4). pp. 225-232. ISSN 0377 9343

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Two secreted antigens of Mycobacterium tuberculosis, namely the antigen 85 complex (30/31) and 38kDa antigens, were purified from the whole culture filtrate by using two dimensional preparative electrophoresis and anion exchange chromatography, respectively. Individual components of the antigen 85 complex namely, antigen 85A, 85B and 85C, were separated using hydrophobic interaction chromatography. The humoral antibody activity to these antigens in sputum positive cases of active pulmonary tuberculosis and normal healthy volunteers was determined by enzyme linked immunosorbent assay (ELISA) and immunoblot. Recombinant 38kDa and antigen 6 were used as reference antigens for the assay. None of the healthy volunteers reacted with the 38kDa antigen, while 52% of the TB sera reacted with it. Of the three components of the antigen 85 complex, 85B gave the highest positivity of 40 per cent. The results of combination of 38kDa with antigen 6 offered better results with 76% positivity.

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Item Type: Article
Uncontrolled Keywords: Antigen 85 complex, ELISA, Immunoblot, M. tuberculosis; 38kDa, Preparative electrophoresis, Hydrophobic interaction chromatography
Subjects: Tuberculosis > Laboratory Research > Immunological
Divisions: Basic Science Research > Immunology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 11 Nov 2013 10:57
Last Modified: 10 Mar 2016 04:46

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