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Standardisation and evaluation of DNA-lanthanide fluorescence spectroscopy for determining rifampicin resistance in Mycobacterium tuberculosis clinical isolates

Mani, C and Selvakumar, N and Gajendiran, N and Panigrahi, B and Venkatesan, P and Narayanan, P R (2003) Standardisation and evaluation of DNA-lanthanide fluorescence spectroscopy for determining rifampicin resistance in Mycobacterium tuberculosis clinical isolates. International Journal of Tuberculosis and Lung Disease, 7 (9). pp. 873-878. ISSN Print: 1027-3719; Online: 1815-7920

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Abstract

SETTING: Tuberculosis Research Centre, Chennai. OBJECTIVE: To rapidly identify multidrug-resistant Mycobacterium tuberculosis using a novel method. DESIGN: A new assay, based on DNA-lanthanide fluorescence, was standardised and evaluated using 93 each of coded rifampicin-resistant and rifampicin-sensitive M. tuberculosis clinical isolates for the correct identification of rifampicin resistance. The results obtained by the new assay were compared with the conventional results. RESULTS AND CONCLUSION: The new assay gave a sensitivity and specificity of 88% and 85%, respectively. It is simple, easy to perform and requires 48 hours for the drug susceptibility results to be available after obtaining the primary culture.

NIRT Creators:
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Item Type: Article
Uncontrolled Keywords: M. tuberculosis; rifampicin resistance; early detection
Subjects: Tuberculosis > Laboratory Research > Bacteriological
Divisions: Basic Science Research > Bacteriology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 12 Nov 2013 10:24
Last Modified: 09 Mar 2016 10:54
URI: http://eprints.nirt.res.in/id/eprint/602

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