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Comparison of DNA sequencing, PCR-SSCP and PhaB assays with indirect sensitivity testing for detection of rifampicin resistance in Mycobacterium tuberculosis

Mani, C and Selvakumar, N and Kumar, V and Narayanan, S and Narayanan, P R (2003) Comparison of DNA sequencing, PCR-SSCP and PhaB assays with indirect sensitivity testing for detection of rifampicin resistance in Mycobacterium tuberculosis. International Journal of Tuberculosis and Lung Disease, 7 (7). pp. 652-659. ISSN Print: 1027-3719; Online: 1815-7920

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Abstract

SETTING: Tuberculosis Research Centre, Chennai, India. OBJECTIVE: To rapidly identify multidrug-resistant Mycobacterium tuberculosis using phenotypic and genotypic methods. DESIGN: Two genotypic assays, DNA sequencing and polymerase chain reaction single strand conformation polymorphism (PCR-SSCP), and one phenotypic assay, phage amplified biological assay (PhaB) were standardised in-house and performed on coded 101 rifampicinresistant and 100 rifampicin-sensitive M. tuberculosis clinical isolates for the identification of rifampicin resistance. RESULTS AND CONCLUSION: The results obtained using the three assays were compared with those from the conventional indirect sensitivity test. The sensitivities and specificities of DNA sequencing, PCR-SSCP and PhaB were 97% and 100%, 76% and 100%, and 97% and 84%, respectively. DNA sequencing was found to be more sensitive and specific than the other tests.

NIRT Creators:
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Item Type: Article
Uncontrolled Keywords: Mycobacterium tuberculosis; rifampicin resistance; DNA sequencing; PCR-SSCP; PhaB
Subjects: Tuberculosis > Laboratory Research > Bacteriological
Divisions: Basic Science Research > Bacteriology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 12 Nov 2013 10:28
Last Modified: 09 Mar 2016 10:55
URI: http://eprints.nirt.res.in/id/eprint/603

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